Effect of hypoxia-ischemia on the expression of feerroportin 1 in O-2A progenitor cells

doi:10.3969/j.issn.0529-1356.2013.03.006

Abstract

Abstract:

Objective To investigate the FPN1 expression and its role in O-2A progenitor cells after hypoxic-ischemic injury.
Methods O-2A progenitor cells were cultured in vitro, indentified with A2B5 antibody and investigated by the localization of
FPN1. Hypoxic-ischemic cell models were established by using the oxygen-glucose deprivation (OGD) method and the cell viability
was assessed by the CCK-8 method. The expression of FPN1 in O-2A progenitor cells after hypoxia-ischemia was detected by
immunofluorescent staining, quantitative real-time polymerase chain reaction analysis and Western blotting analysis. Results
FPN1 was localized at the cell membrane, and in the cytoplasm and processes of O-2A progenitor cells. The cell viability decreased
with time-dependence after 3hours, 6hours, 12hours and 24hours of OGD (P＜0.05). The FPN1 immunofluorescence intensity of O-2A
progenitor cells decreased progressively within 12hours of OGD. The FPN1 mRNA and protein levels downregulated with time-dependence
after 3hours, 6hours and 12hours of OGD (P＜0.05). Conclusion The level of FPN1 expression is down-regulated and cell viability
decreased significantly with time-dependence after OGD, which suggests that FPN1 may play a role in the hypoxic-ischemic injury of
O-2A progenitor cells.

Key words: Ferroportin 1, Hypoxia-ischemia, Real-time PCR, Western blotting, Oligodendrocyte-type-2 astrocyte progenitor cell

LIN Qing ZHANG Geng QIU Rong-hui WANG Wei*. Effect of hypoxia-ischemia on the expression of feerroportin 1 in O-2A progenitor cells[J]. AAS, 2013, 44(3 ): 324-329.

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